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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
11:31

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Published on: July 4, 2018

Mast cell degranulation breaks peripheral tolerance.

V C de Vries1, A Wasiuk, K A Bennett

  • 1Department of Microbiology and Immunology, Dartmouth Medical School and Norris Cotton Cancer Center, Lebanon, NH, USA.

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
|August 18, 2009
PubMed
Summary
This summary is machine-generated.

Mast cells (MCs) regulate transplant tolerance via regulatory T-cells (Tregs). MC degranulation disrupts Treg function, leading to acute transplant rejection and inflammation, highlighting allergy

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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis
16:01

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

Published on: January 26, 2015

Area of Science:

  • Immunology
  • Transplantation immunology
  • Allergy and inflammation

Background:

  • Mast cells (MCs) and regulatory T-cells (Tregs) have a reciprocal relationship in controlling inflammation.
  • MCs mediate Treg-dependent allograft tolerance in skin and cardiac transplants.
  • Tregs mitigate IgE-mediated MC degranulation.

Purpose of the Study:

  • To investigate the impact of MC degranulation on established allograft tolerance.
  • To elucidate the mechanisms by which MCs influence Treg function and distribution.
  • To understand the role of MCs in the breakdown of peripheral tolerance.

Main Methods:

  • Utilized an allograft tolerance model in mice.
  • Induced systemic and intragraft MC degranulation.
  • Assessed Treg suppressor activity, tissue distribution, and molecular expression.
  • Monitored allograft rejection and T-cell dependent inflammation.

Main Results:

  • MC degranulation caused a transient loss of Treg suppressor activity.
  • Tregs rapidly emigrated from the graft following MC degranulation.
  • MCs accumulated in regional lymph nodes, impairing Treg suppressor molecules.
  • MC degranulation led to acute, T-cell dependent rejection of tolerant allografts.

Conclusions:

  • MC degranulation triggers the breakdown of established peripheral allograft tolerance.
  • Allergic reactions involving MC degranulation can transiently reverse Treg-mediated immunosuppression.
  • This mechanism explains how allergy can precipitate acute T-cell mediated inflammation and graft rejection.