Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Are cross-regulatory interactions between homoeotic genes functionally significant?

A González-Reyes1, N Urquia, W J Gehring

  • 1Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.

Nature
|March 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developmental analysis of some mutants of the bithorax system ofDrosophila.

Wilhelm Roux's archives of developmental biology·2017
Same author

Behaviour in aggregates of irradiated imaginal disk cells ofDrosophila.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Development of the Deformed protein pattern in the embryo of the honeybee Apis mellifera L. (Hymenoptera).

Roux's archives of developmental biology : the official organ of the EDBO·2017
Same author

Psychiatric inpatient care: where do we go from here?

Epidemiology and psychiatric sciences·2015
Same author

Jak/Stat signalling regulates dpp transcription to control germline stem cell maintenance in the Drosophila ovary.

Journal of stem cells & regenerative medicine·2014
Same author

Cell competition may function either as tumour-suppressing or as tumour-stimulating factor in Drosophila.

Oncogene·2013

Homoeotic gene interactions in Drosophila development are crucial for segmental identity. However, disrupting the down-regulation of Antennapedia (Antp) and Ultrabithorax (Ubx) genes showed no phenotypic consequences in the epidermis, challenging their role in specifying segment identity.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Segmental identity in Drosophila larvae is determined by homoeotic genes.
  • These genes interact at the transcriptional level, regulating each other's expression.
  • Key interactions include Ultrabithorax (Ubx) down-regulating Antennapedia (Antp), and abdominal-A (abd-A) and Abdominal-B (Abd-B) down-regulating Ubx.

Purpose of the Study:

  • To investigate the functional significance of homoeotic gene cross-regulatory interactions.
  • To assess whether the down-regulation of Antp and Ubx is essential for specifying segmental identity in the epidermis.

Main Methods:

  • Expressing Antennapedia (Antp), Ultrabithorax (Ubx), or both genes under a heat-shock promoter in Drosophila.
  • Analyzing the phenotypic consequences of altered gene expression in the larval epidermis.

Related Experiment Videos

Main Results:

  • Forced expression of homoeotic genes led to their maximal expression in regions where they are normally down-regulated.
  • Interruption of the normal down-regulation of Antp and Ubx did not result in any observable phenotypic changes in the larval epidermis.
  • Homoeotic gene products escaped normal regulatory controls but did not alter segmental identity in the epidermis.

Conclusions:

  • The study challenges the established view that cross-regulatory interactions between homoeotic genes are critical for determining segmental identity in the Drosophila epidermis.
  • The findings suggest that other mechanisms may be responsible for specifying segment identity, or that these interactions are redundant in this context.