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Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
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Introduction to Hemostasis01:05

Introduction to Hemostasis

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Formation of the Platelet Plug01:22

Formation of the Platelet Plug

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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
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Thrombin generation in trauma patients.

Nancy M Dunbar1, Wayne L Chandler

  • 1Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA.

Transfusion
|August 18, 2009
PubMed
Summary
This summary is machine-generated.

Trauma patients with acute coagulopathy of trauma (ACT) exhibit dysregulated hemostasis. Studies show excessive thrombin generation due to procoagulants and reduced inhibitors, increasing bleeding risk.

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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
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09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Area of Science:

  • Hematology
  • Trauma Medicine
  • Hemostasis and Thrombosis

Background:

  • Trauma patients face risks of acute coagulopathy of trauma (ACT), a condition linked to poor outcomes.
  • ACT shares similarities with disseminated intravascular coagulation (DIC) but remains incompletely understood.

Purpose of the Study:

  • To investigate the hemostatic mechanisms in trauma patients with suspected ACT using thrombin generation assays.
  • To compare thrombin generation patterns in trauma patients with ACT, normal subjects, and warfarin-treated patients.

Main Methods:

  • Thrombin generation assays were performed on plasma from 42 trauma patients, 25 normal subjects, and 45 warfarin patients.
  • Laboratory experiments included factor-reduced plasma and diluted plasma to isolate specific hemostatic variables.

Main Results:

  • Trauma patients with ACT showed significantly shorter lag times and higher peak thrombin generation compared to normal subjects.
  • These patients also had lower platelet counts, fibrinogen, and Factor II levels, alongside decreased antithrombin levels.
  • In contrast to factor deficiency, diluted plasma and trauma plasma showed increased thrombin generation with prolonged termination times.

Conclusions:

  • Thrombin generation studies reveal dysregulated hemostasis in trauma patients with ACT.
  • This dysregulation involves excessive systemic thrombin generation driven by procoagulants and insufficient inhibition due to reduced antithrombin levels.