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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Drug Nomenclature01:17

Drug Nomenclature

During the development of a new pharmaceutical, the manufacturer initially assigns a code name to the drug. Once approved, the drug receives a United States Adopted Name (USAN)—a generic, nonproprietary designation. Upon being listed in the United States Pharmacopeia, this nonproprietary name becomes the drug's official name. Additionally, the manufacturer assigns a proprietary name or trademark, which serves as the brand name under which the drug is marketed. It is worth noting that the same...

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Related Experiment Video

Updated: Jun 21, 2026

Designing a Bio-responsive Robot from DNA Origami
13:32

Designing a Bio-responsive Robot from DNA Origami

Published on: July 8, 2013

SNAD: Sequence Name Annotation-based Designer.

Igor A Sidorov1, Denis A Reshetov, Alexander E Gorbalenya

  • 1Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, Netherlands. i.a.sidorov@lumc.nl

BMC Bioinformatics
|August 18, 2009
PubMed
Summary

Researchers can now automatically convert sequence unique identifiers (UIDs) into meaningful names using SNAD (Sequence Name Annotation-based Designer). This tool streamlines data interpretation and enhances scientific communication.

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Last Updated: Jun 21, 2026

Designing a Bio-responsive Robot from DNA Origami
13:32

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Published on: July 8, 2013

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07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Biological sequence data relies on unique identifiers (UIDs) for management.
  • Manual conversion of UIDs to biologically meaningful names is time-consuming and error-prone.
  • Efficient knowledge dissemination requires user-friendly naming conventions.

Purpose of the Study:

  • To develop an automated tool for converting sequence UIDs into biologically meaningful names.
  • To facilitate the utilization and dissemination of sequence-based knowledge.
  • To improve the efficiency of scientific communication.

Main Methods:

  • Introduction of SNAD (Sequence Name Annotation-based Designer).
  • Automated conversion of sequence UIDs from various sources (alignments, trees, text lists).
  • Utilizes precompiled or user-defined templates referencing external database annotations.

Main Results:

  • SNAD enables automatic conversion of sequence UIDs to meaningful names and acronyms.
  • Demonstrated utility with practical examples, particularly in virology.
  • The tool leverages existing sequence annotation data for accurate naming.

Conclusions:

  • A controllable, annotation-based tool for UID conversion has been developed.
  • SNAD enhances the link between sequence annotation quality and communication efficiency.
  • The tool supports better knowledge dissemination among researchers.