Related Experiment Videos
Anti-inflammatory activity of ethanol extract from Geranium sibiricum Linne
Jae-Uoong Shim1, Phil-Sun Oh, Kye-Taek Lim
1Molecular Biochemistry Laboratory, Biotechnology Research Institute & Center for the Control of Animal Hazards Using Biotechnology (BK21), Chonnam National University, Gwangju City, South Korea.
Ethnopharmacological Relevance:
Geranium sibiricum (Geraniaceae) Linne (GSL) is used to heal various disorders of the diarrhea and the intestinal inflammation as an herbal agent in East Asia.
Aims Of The Study:
The purpose of the present study is to determine whether the ethanol (EtOH) extract of GSL regulates the inflammatory reaction stimulated by phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI) in human mast cells (HMC-1).
Materials And Methods:
Western blot was used for activation of mitogen activated protein kinase (MAPK), transcription factors, induced nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 proteins. EMSA was for DNA binding activity. RT-PCR was used for gene expression.
Results:
EtOH extract of GSL (EGS) inhibits the expression of extracellular signal-regulated kinase (ERK), one of a MAPK, nuclear transcription factors involving nuclear factor (NF)-kappaB and Activator protein (AP)-1, COX-2 and iNOS. The results indicated that EGS decreased gene expression of interleukin (IL)-1beta and COX-2 in PMACI stimulated HMC-1 cells.
Conclusion:
Hence, we speculate that EGS can use as a potent anti-inflammatory agent for inflammatory allergic diseases.