Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Efficacy of Ayurveda Regimen as an Adjunct to Hydroxyurea in Sickle Cell Disease: Protocol for a Prospective, Randomized, Open-Label, Blinded End Point Exploratory Study.

JMIR research protocols·2026

Same author

Genetic patterns & public health implications of sickle cell anaemia across populations: A systematic review.

The Indian journal of medical research·2026

Same author

Burden of hepatitis B in asymptomatic blood donor population of India: A systematic review & meta-analysis.

The Indian journal of medical research·2024

Same author

A Pilot Seroprevalence Study Suggests Silent Zika virus Transmission in Bhopal Region of Central India.

Indian journal of public health·2024

Same author

Time Trend and Prevalence Analysis of Transfusion Transmitted Infections among Blood Donors: A Retrospective Study from 2001 to 2016.

Indian journal of community medicine : official publication of Indian Association of Preventive & Social Medicine·2023

Same author

Strengthening quality management system: An assessment of awareness and knowledge in trainees in blood bank.

Asian journal of transfusion science·2022

Same journal

Therapeutic plasma exchange and immunomodulatory strategies in post-infectious syndromes: A review of immune dysregulation in PTLDS, long COVID, ME/CFS, and PANS/PANDAS.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Same journal

Therapeutic plasma exchange in Alzheimer's disease: From clinical trial to real-world evidence.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Same journal

Successful treatment of pure red cell aplasia with Orelabrutinib following major ABO-incompatible hematopoietic stem cell transplantation for acute myeloid leukemia: A case report.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Same journal

Technological advances in selective plasma adsorption: The MTx.100 column and the emergence of subtractive precision medicine.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Same journal

What's happening? Editorial comment: Insights on artificial intelligence, cell therapy and stem cell transplantation safety.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

Same journal

Red blood cell transfusion in elderly patients: Hemoglobin thresholds and clinical outcomes.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

The platelet storage lesion.

Manisha Shrivastava¹

¹Department of Transfusion Medicine, Bhopal Memorial Hospital and Research Centre, Bhopal 462038, MP, India. manishasdr@gmail.com

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis

|August 18, 2009

Summary

This summary is machine-generated.

Platelet storage quality is crucial due to bacterial contamination and storage lesion, limiting shelf life. This review explores current understanding and novel strategies to improve platelet viability and transfusion effectiveness.

More Related Videos

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Jun 20, 2026

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Area of Science:

Transfusion Medicine
Hematology
Biotechnology

Background:

Increasing demand for platelet transfusions requires robust quality standards.
Bacterial contamination and platelet storage lesion (PSL) limit platelet shelf life to 5 days.
Existing knowledge on PSL and platelet health is extensive, but in vivo correlation remains unclear.

Purpose of the Study:

To review the current understanding of platelet storage lesion (PSL).
To explore novel approaches for mitigating PSL and improving platelet quality.
To address the need for better standards in platelet quality assessment during storage.

Main Methods:

Literature review of existing studies on platelet storage lesion.
Analysis of current in vitro assays for platelet quality.
Exploration of emerging strategies to counteract storage-induced platelet degradation.

Main Results:

Platelet storage lesion significantly impacts platelet function and viability.
Current in vitro assays do not fully predict in vivo platelet recovery and survival.
Novel approaches show promise in preserving platelet quality during storage.

Conclusions:

Standardizing platelet quality assessment is essential for safe and effective transfusions.
Further research is needed to correlate in vitro findings with in vivo outcomes.
Developing methods to negate the platelet storage lesion can extend shelf life and improve patient outcomes.