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Plasma cell-free DNA levels in children on peritoneal dialysis

O Ozkaya1, K Bek, A Bedir

  • 1Department of Pediatric Nephrology, Ondokuz Mayis University, School of Medicine, Samsun, Turkey. ozanozkaya@yahoo.com

Nephron. Clinical Practice
|August 18, 2009
PubMed

Insights

Children undergoing peritoneal dialysis (PD) show elevated cell-free DNA (cfDNA) levels compared to healthy controls. This finding suggests cfDNA may be a biomarker in pediatric PD patients, though further research is needed.

Area of Science:

  • Pediatric Nephrology
  • Molecular Diagnostics
  • Biomarker Discovery

Background:

  • Cell-free DNA (cfDNA) is elevated in various conditions, including cancer and autoimmune disorders.
  • Previous studies noted increased cfDNA during hemodialysis, but data in pediatric peritoneal dialysis (PD) patients is limited.

Purpose of the Study:

  • To investigate plasma cell-free DNA (cfDNA) levels in children undergoing peritoneal dialysis (PD).

Main Methods:

  • Plasma cfDNA was quantified using real-time quantitative PCR for the beta-globin gene.
  • The study included 21 children on PD and 21 healthy children.

Main Results:

  • Children on PD exhibited significantly higher median plasma cfDNA concentrations (2,205 genome-equivalents/ml) than healthy controls (1,033 genome-equivalents/ml).
  • A significant positive correlation was found between C-reactive protein levels and plasma cfDNA concentrations in PD patients.

Conclusions:

  • This study is the first to demonstrate elevated cfDNA levels in children on PD treatment.
  • The underlying mechanisms and clinical significance of increased cfDNA in pediatric PD patients require further investigation.
Abstract

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