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[Tyrosinase inhibitor from Angelica dahurica roots]
1Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China. xlpiao@edu.cn
Objective:
To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.
Method:
A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.
Result:
An extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.0907 mmol x L(-1)) with IC50 value of 0.0086 mmol x L(-1). Therefore, it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica.
Conclusion:
The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.