Protein carbonylation in skeletal muscles: impact on function
Esther Barreiro1, Sabah N A Hussain
1Pulmonology Department, IMIM-Hospital del Mar, Catalonia, Spain .
Antioxidants & Redox Signaling
|August 19, 2009
Summary
Oxidative stress in skeletal muscles increases protein carbonylation, impairing muscle function. Further research is needed to understand how this impacts muscle contractile deficit in various diseases.
Area of Science:
- Muscle physiology
- Oxidative stress research
- Biochemistry
Background:
- Reactive oxygen species (ROS) at low levels are crucial for skeletal muscle signaling.
- Elevated ROS production exceeding antioxidant capacity causes oxidative stress and muscle dysfunction.
- Oxidative stress is linked to impaired muscle performance in inflammatory and lung diseases.
Purpose of the Study:
- To investigate the role of oxidant-derived posttranslational protein modifications, specifically protein carbonylation, in skeletal muscle function.
- To determine the functional importance of protein carbonylation in muscle-specific proteins.
- To elucidate the contribution of carbonylation-induced protein dysfunction to overall muscle contractile deficit in pathologies.
Main Methods:
- Observational studies analyzing protein carbonylation levels in skeletal muscle fibers.
- Examination of various animal models with muscle dysfunction.
- Analysis of human subjects with impaired skeletal muscle contractility.
Main Results:
- Elevated levels of protein carbonylation observed in skeletal muscle fibers during oxidative stress.
- Identified carbonylation in key muscle proteins including myofilament, mitochondrial, and cytosolic proteins.
- Carbonylated proteins found in animal models and humans with muscle dysfunction.
Conclusions:
- Protein carbonylation is a significant posttranslational modification associated with oxidative stress in skeletal muscle.
- While carbonylation is prevalent, its precise functional impact on muscle proteins and contribution to contractile dysfunction requires further investigation.
- Understanding carbonylation's role is crucial for addressing muscle pathologies.
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