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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

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Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
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DNA deformations near charged surfaces: electron and atomic force microscopy views.

F G A Faas1, B Rieger, L J van Vliet

  • 1Department of Imaging Science and Technology Delft University of Technology, Delft, The Netherlands.

Biophysical Journal
|August 19, 2009
PubMed
Summary

DNA flexibility near charged surfaces was studied using microscopy. Adhesion causes static bending without affecting dynamic behavior, impacting gene regulation.

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Area of Science:

  • Molecular Biology
  • Biophysics
  • Surface Science

Background:

  • DNA's role as a cellular structural element influencing gene expression.
  • Interaction of DNA with regulatory proteins is crucial for gene regulation.
  • Understanding DNA conformation near surfaces is key to molecular interactions.

Purpose of the Study:

  • To characterize the flexibility of double-stranded DNA (dsDNA) near a charged surface.
  • To investigate the impact of surface adhesion on DNA structural properties.
  • To analyze DNA bending and flexibility using advanced microscopy techniques.

Main Methods:

  • Utilized electron microscopy (EM) and atomic force microscopy (AFM) to image DNA.
  • Employed automated procedures for DNA contour extraction from microscopy data.
  • Applied statistical chain descriptors to analyze DNA conformation and flexibility.

Main Results:

  • Observed unique two-dimensional equilibration of DNA molecules on the substrate surface.
  • EM mounting resulted in decreased DNA persistence length and kurtosis compared to AFM.
  • Local bending analysis confirmed DNA flexibility aligns with the wormlike chain model.
  • Identified additional static bending (kinking) of DNA upon surface adhesion.

Conclusions:

  • Adhesion of DNA to charged surfaces induces static bending without altering dynamic backbone behavior.
  • This kinking phenomenon has implications for DNA-protein interactions and gene regulation.
  • The study provides insights into DNA's structural adaptability in confined environments.