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Updated: Jun 20, 2026

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Multi-dimensional and multi-signal approaches in scanning transmission electron microscopes
1Laboratoire de Physique des Solides (UMR CNRS 8502), Building 510, Université Paris Sud 11, 91405 Orsay, France. colliex@lps.u-psud.fr
Advancements in electron microscopy instrumentation enable atomic-level exploration of nanoworld objects. Improved electron optics and detectors enhance spatial and energy resolution, driving new scientific discoveries.
Area of Science:
- Electron microscopy
- Nanotechnology
- Spectroscopy
Background:
- Instrumentation is crucial for scientific advancement, particularly in electron microscopy.
- Recent progress in hardware and digital data processing has expanded microscopy applications.
- Breakthroughs in electron optics and detectors have significantly improved performance limits.
Purpose of the Study:
- To highlight the impact of recent instrumentation developments in electron microscopy.
- To showcase the extended capabilities for exploring the nanoworld.
- To emphasize the role of enhanced electron energy-loss spectroscopy (EELS) in nanophotonics.
Main Methods:
- Improvements in electron optics, including correctors, filters, and monochromators.
- Advancements in detector efficiency and digital data acquisition/processing.
- Enhanced energy resolution in electron energy-loss spectroscopy (EELS).
Main Results:
- Achieved ultimate atomic-level exploration of nanoworld objects.
- Extended spatial resolution in imaging and sensitivity for single-atom identification.
- Broadened information range with improved EELS energy resolution into the visible spectrum.
- Enabled nanolaboratory device integration with spherical aberration correctors.
Conclusions:
- Modern electron microscopy instrumentation allows unprecedented atomic-scale characterization.
- Enhanced EELS provides powerful insights for nanophotonics development.
- Spherical aberration correctors facilitate advanced in-situ experiments at the nanoscale.
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