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Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Fabrication of Polymer Microspheres for Optical Resonator and Laser Applications

Published on: June 2, 2017

High-Q polymer-coated microspheres for immunosensing applications.

S Soria¹, F Baldini, S Berneschi

¹IFAC-CNR, NelloCarrara Institute of Applied Physics, via Madonna del Piano 10, 50019 Sesto Fiorentino, Florence, Italy. s.soria@ifac.cnr.it

|August 19, 2009

Summary

Polymeric thin layers functionalize silica microspheres for ultrasensitive immunosensors. This method preserves the high quality factor (Q) of resonators, enabling sensitive detection of immunogammaglobulin (IgG).

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Area of Science:

Biomedical Engineering
Materials Science
Analytical Chemistry

Background:

Silica microspheres are utilized as optical resonators for biosensing applications.
Functionalization of microspheres is crucial for immobilizing biomolecules and enhancing sensor performance.
Traditional functionalization methods may impact the optical properties of resonators.

Purpose of the Study:

To explore homogeneous polymeric thin layers as novel functionalizing agents for silica microspherical resonators.
To evaluate poly-L-lactic acid and Eudragit L100 as alternatives to 3-Aminopropyltrimethoxysilane for microsphere functionalization.
To assess the impact of polymeric functionalization on the quality factor (Q) of silica microspheres and their suitability for immunosensor development.

Main Methods:

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Characterization of silica microspheres functionalized with poly-L-lactic acid and Eudragit L100.
Measurement of the quality factor (Q) of bare and functionalized silica microspheres.
Chemical activation and covalent binding of immunogammaglobulin (IgG) to the functionalized microspheres.
Assessment of Q factor changes after IgG immobilization.

Main Results:

Polymeric functionalization with poly-L-lactic acid and Eudragit L100 did not significantly degrade the high quality factor (Q > 10^7) of silica microspheres.
The Q factor of the functionalized microspheres was maintained at approximately 3 x 10^5 after chemical activation and covalent binding of IgG.
The polymeric functionalization strategy is compatible with subsequent biomolecule immobilization.

Conclusions:

Homogeneous polymeric thin layers are effective functionalizing agents for silica microspherical resonators.
This novel functionalization approach preserves the optical integrity of the resonators.
The developed method represents a promising advancement for creating ultra-sensitive immunosensors capable of detecting specific biomolecules like IgG.