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Related Experiment Video

Updated: Jun 20, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Nanodoublers as deep imaging markers for multi-photon microscopy.

Jerome Extermann¹, Luigi Bonacina, Enrique Cuña

¹Université de Genève, GAP-Biophotonics, 20 rue de l'Ecole de Médecine, 1211 Geneva, Switzerland.

|August 19, 2009

Summary

This summary is machine-generated.

We excited iron iodate nanocrystals using two laser wavelengths, demonstrating their potential for deeper biological imaging. This wavelength flexibility enhances the penetration depth of embedded markers.

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Area of Science:

Materials Science
Nanotechnology
Biomedical Imaging

Background:

Non-linear optical (NLO) nanomaterials offer unique optical properties for advanced imaging.
Second-harmonic (SH) generation is a key NLO process used in microscopy and sensing.
Developing NLO nanoparticles with tunable excitation is crucial for overcoming biological tissue limitations.

Purpose of the Study:

To investigate the SH-active properties of iron iodate (Fe(IO3)3) nanocrystals.
To explore the potential of using multiple laser wavelengths for exciting these nanocrystals.
To demonstrate the application of wavelength flexibility for enhanced imaging penetration depth in biological samples.

Main Methods:

Synthesis and characterization of Fe(IO3)3 nanocrystals.
Experimental excitation of SH signals using 800 nm and 1550 nm pulsed lasers.
Complementary experimental and numerical studies to analyze SH generation and penetration depth.

Main Results:

Fe(IO3)3 nanocrystals exhibit efficient SH activity when excited at both 800 nm and 1550 nm.
The non-phase-matched nature of the nanoparticles allows for wavelength flexibility.
Demonstrated increased imaging penetration depth for markers embedded in biological samples by exploiting this flexibility.

Conclusions:

Fe(IO3)3 nanocrystals are promising NLO materials for multimodal excitation.
Wavelength flexibility in SH nanoparticles can be leveraged to improve deep-tissue imaging.
This work opens avenues for advanced bio-imaging applications with enhanced resolution and depth.