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Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine
Published on: October 7, 2014
Studies on selenate and selenite absorption by sheep ileum using an everted sac method and an isolated, vascularly
J C Turner1, P J Osborn, S M McVeagh
1Wallaceville Animal Research Center, Ministry of Agriculture and Fisheries, Upper Hutt, New Zealand.
Abstract:
1. Using everted sacs of sheep ileum, selenate uptake was shown to be more rapid than selenite uptake. 2. Sulphate, thiosulphate and ouabain all inhibited the uptake of selenate. A Na+K(+)-ATPase is probably responsible for energizing the ileal brush-border transport of selenate. 3. The isolated, vascularly perfused ileum was shown to absorb selenate more rapidly than selenite. 4. The perfusion system described would appear to be a useful tool for studying the kinetics of intestinal absorption and the measurement of metabolic transformations within the mucosa.
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