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Effects of adrenergic drugs on intracellular electrical potential difference of rabbit ciliary epithelial cells
Abstract:
The effects of adrenergic drugs on intracellular electrical potential difference (PDI) of rabbit ciliary epithelial cells were investigated. Epinephrine as well as norepinephrine hyperpolarized the PDI at lower concentrations (10(-6) M) and depolarized the PDI at higher concentrations (greater than 10(-5) M). Isoproterenol produced a depolarization of PDI and phenylephrine caused a hyperpolarization only. After pretreatment with propranolol, the change of PDI by isoproterenol was minimal. Selective agonists and antagonists were used to further characterize adrenergic effects on the PDI. Both beta 1 and beta 2 agonists caused a depolarization of PDI while both beta 1 and beta 2 antagonists produced a hyperpolarization. Alpha 1 antagonist depolarized the PDI and alpha 2 antagonist hyperpolarized the PDI. Such electrophysiological effects of the adrenergic drugs confirm the presence of alpha and beta adrenoceptors in the rabbit ciliary epithelial cells.