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Updated: Jun 20, 2026

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
Published on: July 19, 2016
Relative quantum yield measurements of coumarin encapsulated in core-shell silica nanoparticles
Erik Herz1, Thomas Marchincin, Laura Connelly
1Department of Materials Science and Engineering, Cornell University, Ithaca, NY 14853, USA.
Abstract:
Fluorescent silica nanoparticles encapsulating organic fluorophores provide an attractive materials platform for a wide array of applications where high fluorescent brightness is required. We describe a class of fluorescent silica nanoparticles with a core-shell architecture and narrow particle size distribution, having a diameter of less than 20 nm and covalently incorporating a blue-emitting coumarin dye. A quantitative comparison of the scattering-corrected relative quantum yield of the particles to free dye in water yields an enhancement of approximately an order of magnitude. This enhancement of quantum efficiency is consistent with previous work on rhodamine dye-based particles. It provides support for the argument that improved brightness over free dye in aqueous solution is a more general effect of covalent incorporation of fluorescent organic dyes within rigid silica nanoparticle matrices. These results indicate a synthetic route towards highly fluorescent silica nanoparticles that produces excellent probes for imaging, security, and sensing applications.
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