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Updated: Jun 20, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
Published on: August 2, 2015
Interaction trap/two-hybrid system to identify interacting proteins
Erica A Golemis1, Ilya Serebriiskii1, Russell L Finley2
1Fox Chase Cancer Center, Philadelphia, Pennsylvania.
Abstract:
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
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