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Updated: Jun 20, 2026

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
Published on: July 18, 2011
Imaging and studying human topoisomerase I on mica surfaces in air and in liquid by atomic force microscopy
Zhiguo Liu1, Ronghua Meng, Yuangang Zu
1Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin, China.
Abstract:
In this study, the topography of human topoisomerase I (TOPO I) on mica surfaces in air and in liquid has been studied by atomic force microscopy (AFM). The average height of TOPO I on mica surface in air measured by AFM was 2.59+/-0.32 nm. After adsorption of the 0.3 U/microl TOPO I on mica surfaces for 2 h, and then imaged in liquid by AFM, well-separated single TOPO I was observed. The average height of TOPO I on mica surfaces in liquid measured by AFM was 2.93+/-0.42 nm. After adsorption of the 4 U/microl TOPO I on mica surfaces for 1.5 h, TOPO I monolayer can be formed. The produced TOPO I monolayer on mica was flat and exhibited good stability.
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