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[Effects of resveratrol on nuclear factor-kappaB and pro-inflammatory factors from lipopolysaccharide activated

Shen Zhang1, Shuping Li, Taotao Wei

  • 1Department of Laboratory Medicine, Huaihua Medical College, Huaihua 418000, China. zhang123shen@163.com

Wei Sheng Yan Jiu = Journal of Hygiene Research
|August 20, 2009
PubMed
Abstract

Insights

Resveratrol (Res) inhibits lipopolysaccharide (LPS)-induced activation of nuclear factor-kappaB (NF-kappaB) and inflammatory cytokine expression in macrophages. This suggests Res can modulate inflammatory responses in macrophages.

Area of Science:

  • Immunology
  • Molecular Biology
  • Pharmacology

Background:

  • Lipopolysaccharide (LPS) is a potent activator of inflammatory responses.
  • Nuclear factor-kappaB (NF-kappaB) is a key transcription factor regulating inflammation.
  • Cytokines like TNF-alpha, IL-1beta, and IL-6 play critical roles in inflammatory processes.

Purpose of the Study:

  • To investigate the effect of resveratrol (Res) on LPS-induced NF-kappaB activation.
  • To examine Res's impact on inflammatory cytokine expression in macrophages.

Main Methods:

  • Murine peritoneal macrophages were stimulated with LPS alone or with LPS and Res.
  • NF-kappaB activity was assessed using electrophoretic mobility shift assay (EMSA).
  • Cytokine (TNF-alpha, IL-1beta, IL-6) expression was quantified by RT-PCR and ELISA.

Main Results:

  • LPS stimulation significantly increased NF-kappaB activity and cytokine levels from 6 to 12 hours.
  • Resveratrol significantly reduced NF-kappaB activity and TNF-alpha, IL-1beta, and IL-6 levels compared to LPS alone.

Conclusions:

  • LPS activates NF-kappaB and upregulates inflammatory cytokine genes in macrophages.
  • Resveratrol inhibits LPS-induced NF-kappaB activation and modulates the expression of key inflammatory cytokines.

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