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Related Experiment Video

Updated: Jun 20, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Single-quantum dot imaging with a photon counting camera.

X Michalet1, R A Colyer, J Antelman

  • 1Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA. michalet@chem.ucla.edu

Current Pharmaceutical Biotechnology
|August 20, 2009
PubMed
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Researchers developed a novel photon-counting detector for ultra-sensitive single-molecule imaging. This detector enhances biomolecular studies by providing high-resolution data and fluorescence lifetime information.

Area of Science:

  • Biophysics
  • Optical Imaging
  • Biotechnology

Background:

  • Single-molecule fluorescence imaging is crucial for studying biomolecular activity and cellular processes.
  • Advancements in fluorescent probes and detector sensitivity have driven progress in this field.
  • Existing detectors have limitations in capturing comprehensive single-molecule data.

Purpose of the Study:

  • To introduce a new wide-field, photon-counting detector designed for ultra-sensitive single-molecule imaging.
  • To enable the acquisition of high temporal and spatial resolution data for each photon.
  • To provide additional information such as fluorescence lifetime and spatio-temporal correlations.

Main Methods:

  • Development of a novel wide-field, photon-counting detector.

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Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
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Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

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Last Updated: Jun 20, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection
12:57

Resonance Fluorescence of an InGaAs Quantum Dot in a Planar Cavity Using Orthogonal Excitation and Detection

Published on: October 13, 2017

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

  • Utilizing quantum dots to demonstrate single-molecule imaging capabilities.
  • Characterizing detector performance in terms of resolution and sensitivity.

    • Main Results:

    • The detector achieves ultra-sensitive single-molecule imaging.
    • It provides high temporal and spatial resolution per photon.
    • Demonstrated performance with quantum dots for imaging applications.

    Conclusions:

    • The new detector significantly advances single-molecule imaging capabilities.
    • It offers a versatile platform for various biological and chemical research applications.
    • Future developments aim to further enhance detector performance and expand its applications.