Dependence of retinogeniculate transmission in cat on NMDA receptors

1. We have examined the possibility that N-methyl-D-aspartate (NMDA) receptors may be involved in the visual response of relay cells in the cat dorsal lateral geniculate nucleus (dLGN). The selective NMDA receptor antagonists D-2-amino-5-phosphonovalerate (APV) and 3-[(+/-)-2-carboxypiperazin-4-yl]-propyl-1-phosphonic acid (CPP) have been iontophoretically applied to X and Y cells in the dLGN and their effects on the visual response to a light spot flashed within the receptive field center determined. 2. The antagonist effects were assessed at ejection current levels producing a selective blockade of the responses to iontophoretically applied NMDA with respect to those elicited by the non-NMDA receptor agonists quisqualate and kainate. These selective effects were determined in an experimental paradigm where the visual response and responses to NMDA and the non-NMDA receptor agonists were compared in the same test run. The data refer to a total population of 52 cells (28 X, 24 Y). 3. Application of APV abolished or greatly reduced the visual responses of both X and Y cells. The mean percentage reduction in the visual response for the X cells studied was 59 +/- 10% (SE; n = 7) and for the Y cells 66 +/- 8% (SE; n = 11). Both the early onset transient and the sustained component of the visual response to the flashed stimulus were equally affected. 4. The antagonist CPP produced a similar pattern of effect to APV, substantially reducing or abolishing the visual response in both X and Y cells.(ABSTRACT TRUNCATED AT 250 WORDS)