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Published on: February 8, 2016
Chemoporation using saponins or cholates: an alternative method for transformation of bacterial cells
Matjaz Ravnikar1, Andreja Irman, Natasa Radić
1Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia. matjaz.ravnikar@ffa.uni-lj.si
Abstract:
A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding of bacterial transformation.
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