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Related Experiment Video

Updated: Jun 20, 2026

Production and Purification of Baculovirus for Gene Therapy Application
06:11

Production and Purification of Baculovirus for Gene Therapy Application

Published on: April 9, 2018

Concanavalin A affinity chromatography for efficient baculovirus purification.

Guan-Yu Chen1, Chi-Yuan Chen, Margaret Dah-Tsyr Chang

  • 1Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.

Biotechnology Progress
|August 20, 2009
PubMed
Summary

This study introduces concanavalin A (Con A) chromatography for purifying baculoviruses, a key step for gene therapy and vaccines. This method effectively removes impurities, yielding high-quality viral vectors for in vivo applications.

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Area of Science:

  • Biotechnology
  • Virology
  • Biochemistry

Background:

  • Baculovirus is a promising vector for gene delivery and vaccines, with increasing in vivo applications driving demand for purified virus.
  • The baculoviral envelope protein gp64 is a glycoprotein, suggesting potential for affinity-based purification methods.

Purpose of the Study:

  • To develop a simple and effective baculovirus purification process using concanavalin A (Con A) chromatography.
  • To assess the stability and recovery of baculovirus during purification.

Main Methods:

  • Concanavalin A (Con A) chromatography was employed, leveraging the affinity between baculovirus gp64 and Con A.
  • Virus stability and recovery were quantified using transducing titers (TT) and viral particles (VP).
  • Diafiltration with tangential flow filtration was used, followed by Con A column binding, washing, and elution with alpha-D-methylmannoside.

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Last Updated: Jun 20, 2026

Production and Purification of Baculovirus for Gene Therapy Application
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Main Results:

  • Diafiltration yielded approximately 75% recovery in TT and 82% in VP, with baculovirus stability sensitive to buffer conditions.
  • Con A chromatography demonstrated strong binding, with wash steps removing >99% of protein impurities.
  • Elution with 0.6 M alpha-D-methylmannoside resulted in ~16% VP and ~15.3% TT recovery, achieving a low VP/TU ratio of 41.4.

Conclusions:

  • Concanavalin A chromatography is suitable for simple and effective baculovirus purification.
  • This method yields high-quality purified baculovirus suitable for in vivo applications.
  • The Con A chromatography approach may be applicable to purifying other viruses displaying surface glycoproteins.