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Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
Published on: February 27, 2021
Comparative sequence analysis and serological and infectivity studies indicate that cocksfoot mild mosaic virus is a
Angelika Ziegler1, Graham Cowan, Lesley Torrance
1Plant Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK. aziegl@scri.ac.uk
Abstract:
The complete nucleotide sequence of the Phleum isolate of cocksfoot mild mosaic virus (CMMV-P) and the coat protein sequence of the cocksfoot isolate (CMMV-1) were determined. Comparative sequence analysis revealed a close relationship with Panicum mosaic virus (PMV; genus Panicovirus), and together with serological studies, the work supports the classification of CMMV in the family Tombusviridae, genus Panicovirus rather than, as is currently proposed, the genus Sobemovirus. A full-length cDNA clone was prepared, and RNA transcripts synthesised from cDNA were shown to be infectious when inoculated to Hordeum vulgare.
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