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Updated: Jun 20, 2026
Lentiviral Vector-mediated Gene Therapy of Hepatocytes Ex Vivo for Autologous Transplantation in Swine
Published on: November 4, 2018
[Construction of adeno-associated virus vector containing ANG-1 gene and its expression in pig mesenchymal stem
Cheng-chu ZHU1, Shi-lin CHEN, Yu-qing LIU
1Department of Cardiothoracic Surgery, Taizhou Hospital, Linhai 317000, China.
Objective:
To construct recombinant adeno-associated virus (rAAV) vector containing angiopoietin-1 (ANG-1) gene and to express the ANG-1 in targeting cells.
Methods:
ANG-1 cDNA was obtained from human spleen by RT-PCR and was inserted into AAV vectors to form rAAV ANG-1, the virus stocks in high titer were harvested. The rAAVANG-1 and rAAV GFP were transferred into pig mesenchymal stem cells and the expression of ANG-1 was detected by Western blot.
Results:
The cloned ANG-1 cDNA was 1515bp in length which was in accordance with that reported previously. Titration of rAAVANG-1 stock was 9 X 10(11)v.g/ml. The expression of ANG-1 gene was detected in transfected cells. Forty-eight hours after rAAV GFP was transfected into mesenchymal stem cells, 55% cells expressed GFP.
Conclusion:
The constructed rAAV ANG-1 vector has successfully transfered and expressed in pig mesenchymal stem cells.
