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Related Experiment Videos

Two distinct cDNAs for human IMP dehydrogenase.

Y Natsumeda1, S Ohno, H Kawasaki

  • 1Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Japan.

The Journal of Biological Chemistry
|March 25, 1990
PubMed
Summary

Two types of human IMP dehydrogenase were identified, with differing mRNA expression in normal leukocytes versus ovarian tumors. This discovery may impact antileukemic chemotherapy drug development.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Inosine monophosphate (IMP) dehydrogenase (EC 1.1.1.205) is crucial for GTP biosynthesis and a key target in leukemia treatment.
  • Understanding the molecular forms of IMP dehydrogenase is essential for developing effective therapies.

Purpose of the Study:

  • To isolate and characterize distinct human IMP dehydrogenase (IMPDH) cDNA clones.
  • To investigate the differential expression of IMPDH mRNA in normal and cancerous human tissues.

Main Methods:

  • Isolation of cDNA clones encoding IMP dehydrogenase from a human spleen library.
  • Northern hybridization analysis of poly(A)+ RNA from human leukocytes and ovarian tumors.

Main Results:

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  • Two distinct cDNA clones (type I and type II) encoding IMP dehydrogenase were isolated.
  • Both clones encode highly similar proteins (514 residues, 84% identity).
  • Type I IMPDH mRNA is predominant in normal leukocytes, while type II predominates in ovarian tumors.

    • Conclusions:

    • This study reports the first evidence of two distinct molecular species of human IMP dehydrogenase.
    • The differential expression suggests varying sensitivities to IMPDH-targeted drugs, impacting antileukemic chemotherapy.