Related Experiment Video

Updated: Jun 20, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Genetic diversity of sapovirus infections in Danish children 2005-2007

Christina K Johnsen1, Sofie Midgley, Blenda Böttiger

  • 1Department of Virology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|August 22, 2009
PubMed

Insights

Sapoviruses frequently cause gastroenteritis in young children in Denmark. Genotype I.1 was the most common, though other sapovirus types circulated periodically.

Area of Science:

  • Virology
  • Pediatric Gastroenterology
  • Epidemiology

Background:

  • Sapoviruses are a significant cause of acute gastroenteritis, particularly in pediatric populations.
  • Understanding sapovirus epidemiology is crucial for public health interventions.

Purpose of the Study:

  • To establish a sapovirus collection from Danish children.
  • To investigate the genetic diversity and epidemiology of circulating sapoviruses.

Main Methods:

  • Real-time PCR was used to detect sapoviruses in 1104 children (aged 0-3 years) with acute gastroenteritis over 24 months (2005-2007).
  • Genotyping involved sequencing of the capsid and polymerase genes for positive samples.

Main Results:

  • Sapoviruses were detected in 9% (97/1104) of children, with highest prevalence in the 7-18 month age group.
  • Three genogroups and seven genotypes were identified, with Genotype I.1 found in 50% of positive cases and present throughout the study.
  • While overall no clear seasonality was observed, increased positive sample proportions occurred in September, November, and February.

Conclusions:

  • Sapoviruses are prevalent in children with gastroenteritis in Denmark.
  • Genotype I.1 is the dominant circulating sapovirus, but other genotypes emerge periodically.
  • Further research into sapovirus seasonality and genotype dynamics is warranted.
Abstract

More Related Videos

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR15:16

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Published on: July 22, 2012

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...