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Related Experiment Video

Updated: Jun 20, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes.

Christian Elabd1, Chiara Chiellini, Mamen Carmona

  • 1IBDC, Université de Nice Sophia-Antipolis, CNRS, 06 107 Nice cedex 2, France.

Stem Cells (Dayton, Ohio)
|August 22, 2009
PubMed
Summary

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Human stem cells can be induced to become brown fat cells, which may help combat obesity. This discovery offers a new way to study and potentially treat metabolic disorders by targeting brown adipose tissue.

Area of Science:

  • Biotechnology
  • Metabolic Research
  • Stem Cell Biology

Background:

  • Recent findings indicate active brown adipose tissue in adult humans holds metabolic significance.
  • Human brown fat precursor cells have been unavailable for research until now.
  • Human multipotent adipose-derived stem (hMADS) cells can differentiate into white adipocytes with key properties.

Purpose of the Study:

  • To investigate the potential of hMADS cells to differentiate into brown adipocytes.
  • To identify factors that can induce a switch from white to brown adipocyte phenotype.
  • To establish a human cell model for screening drugs that promote brown adipocyte function.

Main Methods:

  • Chronic exposure of hMADS cell-derived white adipocytes to specific peroxisome proliferator-activated receptor (PPAR) agonists.

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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

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Last Updated: Jun 20, 2026

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
07:22

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Published on: March 28, 2013

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

  • Analysis of gene expression for uncoupling protein one (UCP1) and CIDEA.
  • Measurement of oxygen consumption and cellular uncoupling.
  • Assessment of UCP1 protein expression and its response to beta-adrenergic receptor (AR) agonists.

    • Main Results:

    • Specific PPARgamma agonist treatment induced hMADS cell-derived white adipocytes to express UCP1 and CIDEA mRNA, characteristic of brown adipocytes.
    • This phenotypic switch was accompanied by increased oxygen consumption and uncoupling.
    • UCP1 protein expression was observed and stimulated by beta-AR agonists, including the beta3-AR agonist.
    • hMADS cells provide a viable model for studying human brown adipocyte differentiation and function.

    Conclusions:

    • hMADS cells can be differentiated into functional brown adipocytes under specific conditions.
    • This provides a valuable human cell model for research into brown adipose tissue and metabolic diseases.
    • The findings support the potential development of therapeutic strategies targeting brown adipocytes to manage caloric intake and metabolic health.