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Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Complementary DNA01:44

Complementary DNA

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Cre complementation with variable dimerizers for inducible expression in neurons.

Tomohiko Maruo1, Tatsuhiko Ebihara, Emi Sato

  • 1Department of Cell Biology, School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.

Journal of Medical and Dental Sciences
|August 25, 2009
PubMed
Summary
This summary is machine-generated.

Cre complementation reconstitutes DNA recombinase activity using inactive fragments. Different dimerizing modules show varied efficiency in neuronal cells, enabling targeted gene manipulation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Neuroscience

Background:

  • Cre complementation reconstitutes DNA recombinase activity from inactive fragments.
  • This technique offers potential for spatiotemporal control of gene expression.
  • Limiting Cre fragment overlap restricts activity to specific cell subsets.

Purpose of the Study:

  • To analyze the efficiency of Cre complementation using different dimerizing modules.
  • To evaluate Cre complementation in non-neuronal and primary neuronal cells.
  • To assess the potential of dimerizer-based Cre complementation for inducible gene manipulation in vivo.

Main Methods:

  • Assessed Cre complementation efficiency with three distinct dimerizing modules.
  • Utilized non-neuronal cell cultures for initial efficiency testing.
  • Employed primary hippocampal neurons from transgenic mice with a reporter gene (loxP-flanked).

Main Results:

  • Differential Cre complementation efficiencies were observed across the tested dimerizing modules.
  • This differential activity was confirmed in Cre-dependent recombination assays in primary neurons.
  • The study identified variations in recombination efficiency based on the dimerizer used.

Conclusions:

  • Dimerizer choice significantly impacts Cre complementation efficiency.
  • Cre complementation shows promise for inducible gene expression or inactivation in specific neuronal subsets.
  • This method could be valuable for precise genetic manipulation within complex nervous tissue environments.