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Updated: Jun 20, 2026
Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
Published on: December 12, 2016
[Changes of ICAM-1 mRNA expression in rats' skeletal muscle after contusion]
Ming Pei1, Jun-Hong Sun, Cai-Rong Gao
1School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China. peiming5992@163.com
Objective:
To investigate the expression of intercellular adhesion molecular-1 (ICAM-1) mRNA in contused skeletal muscle of rats using fluorescence in situ hybridization (FISH) and its relationship with the contusion interval.
Methods:
To make the contusion models with rats skeletal muscle. The samples were taken to extract mRNA at 0.5 h, 1 h, 6 h, 12 h, 18 h, 24 h, 30 h and from the control group after contusion respectively. FISH was performed on frozen section samples and the sections were observed using LSCM.
Results:
The expression of ICAM-1 mRNA peaked at 6 h in skeletal muscle after contusion. Its level fell to 3.46 times the level of control group at 18 h and then increased again.
Conclusion:
The time-order expression of ICAM-1 mRNA in 30 hours after contusion is potentially useful for estimation of early wound age.
Insights
The expression of intercellular adhesion molecule-1 (ICAM-1) mRNA in rat skeletal muscle peaks at 6 hours post-contusion. This temporal expression pattern may help estimate early wound age.
Area of Science:
- Molecular biology
- Skeletal muscle research
- Biomarker analysis
Context:
- Skeletal muscle contusions are common injuries.
- Accurate wound age estimation is crucial for forensic and clinical assessments.
- Intercellular adhesion molecule-1 (ICAM-1) is implicated in inflammatory responses.
Purpose:
- To investigate the temporal expression of ICAM-1 mRNA in rat skeletal muscle following contusion.
- To determine the correlation between ICAM-1 mRNA levels and the time elapsed since injury.
- To evaluate the potential of ICAM-1 mRNA as a biomarker for early wound age estimation.
Summary:
- Rat skeletal muscle contusion models were established.
- Intercellular adhesion molecule-1 (ICAM-1) mRNA expression was quantified using fluorescence in situ hybridization (FISH) at various time points post-injury (0.5 h to 30 h).
- ICAM-1 mRNA levels exhibited a peak at 6 hours, followed by a decrease and subsequent increase, showing a distinct temporal profile.
Impact:
- The study reveals a dynamic expression pattern of ICAM-1 mRNA in contused skeletal muscle.
- Findings suggest ICAM-1 mRNA could serve as a valuable indicator for estimating the age of skeletal muscle injuries within the first 30 hours.
- This research contributes to understanding the molecular response to muscle trauma and its forensic applications.
