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Macrophages activation and nitric oxide alterations in mice treated with Pleurotus florida
Tooba Ghazanfari1, Roya Yaraee, Zohre Farahnejad
1Immunoregulation Research Center Shahed University Tehran, Iran. tghazanfari@yahoo.com
Abstract:
Macrophages play an essential role against invading pathogen and malignancies. The present study addresses the in vivo effect of P.florida extract on nitric oxide (NO) production and cell viability of macrophages. Forty Balb/c mice were divided to 8 groups and were treated with different doses of P. florida aqueous extract. MTT test has been performed in order to evaluate viability of intraperitoneal macrophages and Griess method to measure NO production of macrophages. The results indicated that cell viability of macrophages significantly increased by oral administration of P. florida with 200, 500 and 1000mg/kg/day. Also, NO production significantly increased by oral administration of doses of 500 and 1000mg/kg/day of P. florida. but i.p. injection of P. florida with 10,20,50,100mg/kg/day significantly decreased NO production by macrophages. This study showed a macrophage activator function for P. florida and also may confirms its anti inflammatory role. Further studies are needed to address effective phytochemicals of this edible mushroom and their mechanisms.
Insights
This study reveals Pleurotus florida extract boosts macrophage viability and nitric oxide (NO) production when administered orally, suggesting potential anti-inflammatory benefits. Intraperitoneal injection, however, reduced NO levels.
Area of Science:
- Immunology
- Pharmacology
- Mycology
Background:
- Macrophages are crucial immune cells involved in pathogen defense and tumor surveillance.
- Modulating macrophage activity is a key strategy for treating inflammatory diseases and cancer.
Purpose of the Study:
- To investigate the in vivo effects of Pleurotus florida aqueous extract on macrophage nitric oxide (NO) production and cell viability.
- To assess the potential immunomodulatory and anti-inflammatory properties of P. florida.
Main Methods:
- Forty Balb/c mice were divided into 8 groups and treated with varying doses of P. florida extract.
- Macrophage cell viability was assessed using the MTT assay.
- Nitric oxide production was measured using the Griess assay.
Main Results:
- Oral administration of P. florida (200, 500, 1000 mg/kg/day) significantly enhanced macrophage cell viability.
- Oral administration of P. florida (500, 1000 mg/kg/day) significantly increased NO production.
- Intraperitoneal injection of P. florida (10-100 mg/kg/day) significantly decreased NO production.
Conclusions:
- Pleurotus florida exhibits macrophage activator properties.
- The findings suggest a potential anti-inflammatory role for P. florida.
- Further research is warranted to identify active phytochemicals and elucidate their mechanisms of action.
