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Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization
Published on: September 1, 2018
Factor XII gene expression in endometrial stromal cells during decidualisation
Hiroaki Kawato1, Tsutomu Tabata, Hiroyuki Minoura
1Department of Obstetrics and Gynecology, Mie University School of Medicine, Edobashi, Tsu-City, Mie, Japan.
Reproduction, Fertility, and Development
|August 25, 2009
Summary
Factor XII (FXII) gene expression significantly increases during human embryo implantation. This upregulation in decidualised endometrial stromal cells (ESC) suggests FXII
Area of Science:
- Reproductive Biology
- Molecular Endocrinology
- Human Embryology
Background:
- Decidualisation of endometrial stromal cells (ESC) is crucial for human embryo implantation.
- Understanding gene expression changes during decidualisation is key to elucidating implantation mechanisms.
Purpose of the Study:
- To identify genes differentially expressed during ESC decidualisation.
- To investigate the role of Factor XII (FXII) in human embryo implantation.
Main Methods:
- Microarray analysis to compare gene expression in decidualised vs. non-decidualised ESC.
- Quantitative analysis of FXII mRNA in endometrial tissues and in vitro decidualised ESC.
- Western blotting to confirm FXII protein expression.
Main Results:
- Factor XII (FXII) gene expression is significantly upregulated during ESC decidualisation.
- FXII mRNA expression increases from the proliferative to secretory endometrium and is markedly elevated in pregnancy decidua.
- In vitro decidualisation with sex steroids induced a ~25.3-fold increase in FXII mRNA expression in ESC.
Conclusions:
- FXII is upregulated in human endometrial stromal cells during decidualisation.
- The kallikrein-kininogen-kinin system may be activated during human embryo implantation due to increased FXII expression.
