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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Digital Microfluidics for Automated Proteomic Processing
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Published on: November 6, 2009

Microfluidics with MALDI analysis for proteomics--a review.

Jeonghoon Lee1, Steven A Soper, Kermit K Murray

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.

Analytica Chimica Acta
|August 25, 2009
PubMed
Summary
This summary is machine-generated.

This review covers microfluidic devices for proteomic analysis, focusing on their integration with matrix-assisted laser desorption ionization (MALDI) mass spectrometry for real-time or off-line measurements.

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Published on: August 18, 2017

Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Mass Spectrometry

Background:

  • Microfluidic devices are increasingly utilized for proteomic analyses.
  • Many microfluidic systems are engineered for mass spectrometry detection.
  • Integrating microfluidics with mass spectrometry offers enhanced analytical capabilities.

Purpose of the Study:

  • To provide an overview of microfluidic devices for proteomic analysis.
  • To discuss chip fabrication, microfluidic components, and interfacing with MALDI mass spectrometry.
  • To explore various analytical functions integrated with microfluidic-mass spectrometry systems.

Main Methods:

  • Review of existing literature on microfluidic device fabrication and integration.
  • Discussion of microfluidic components relevant to proteomic sample handling.
  • Examination of interfacing strategies between microfluidic devices and MALDI mass spectrometry.

Main Results:

  • Microfluidic devices can be coupled on-line for real-time analysis or used off-line for sample analysis and deposition.
  • Integration enables on-chip sample cleanup, digestion, and separations.
  • Various approaches for combining microfluidics with MALDI-MS are presented.

Conclusions:

  • Microfluidic devices offer versatile platforms for advanced proteomic analyses.
  • The integration of microfluidics with MALDI-mass spectrometry facilitates efficient and sensitive proteomic workflows.
  • These integrated systems support both real-time and off-line analytical strategies.