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Minimal Erythema Dose (MED) Testing

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Effectiveness of different substrate materials for in vitro sunscreen tests.

Denis Garoli¹, Maria Guglielmina Pelizzo, Piergiorgio Nicolosi

¹CNR-INFM LUXOR via Gradenigo, 6B, Padua 35131, Italy.

Journal of Dermatological Science

|August 25, 2009

Summary

This study evaluated various substrates for in vitro sunscreen testing, finding Transpore, roughened quartz, and Vitro-Skin best for SPF assessment due to strong in vitro-in vivo correlation. PMMA, PTFE, and quartz showed good photo-stability.

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Area of Science:

Dermatology
Cosmetic Science
Photobiology

Background:

In vitro measurements are crucial for sunscreen reliability, assessing photoprotection and photo-stability.
Standardized testing methods are needed for accurate sunscreen evaluation.

Purpose of the Study:

To characterize and compare different substrates for in vitro sunscreen testing.
To determine the suitability of various materials for Sun Protection Factor (SPF) evaluation.

Main Methods:

Evaluated roughened PMMA plates, Transpore, Vitro-Skin, roughened quartz, and PTFE plates.
Measured total absorbance of seven sunscreen products with varying SPF values on each substrate.
Assessed substrate photo-stability after UV irradiation.

Main Results:

Transpore, roughened quartz, and Vitro-Skin demonstrated better correlation between in vitro and in vivo SPF measurements.
PMMA, PTFE, and roughened quartz plates exhibited photo-stability post-UV irradiation.
Initial calibration is necessary for sunscreens with different labeled SPF values.

Conclusions:

Transpore, roughened quartz, and Vitro-Skin are recommended for in vitro SPF testing.
While in vitro and in vivo SPF results correlate, they are not identical.
Substrate selection impacts the accuracy of in vitro sunscreen efficacy assessments.