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Updated: Feb 18, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
Determination of glycosylation sites and site-specific heterogeneity in glycoproteins
Hyun Joo An1, John W Froehlich, Carlito B Lebrilla
1Department of Chemistry, University of California, Davis, CA 95616, USA.
Abstract:
Glycosylation is one of the most common post-translational modifications (PTMs) of proteins. At least 50% of human proteins are glycosylated with some estimates being as high as 70%. Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associated with each site. Recent advances have led to the development of new analytical methods that employ mass spectrometry extensively making it possible to obtain the glycosylation site and the site microheterogeneity. These tools will be important for the eventual development of glycoproteomics.
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