Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Managing Acute Cholecystitis in a Patient Who Is Not a Surgical Candidate.

AJR. American journal of roentgenology·2026
Same author

Electrochemical Aptasensing Platform for Culture-Free, Selective Detection of Airborne Bacterial Species.

ACS sensors·2026
Same author

Modified Relative Handgrip Strength and Tooth Loss: A Nationwide Study.

Journal of oral rehabilitation·2025
Same author

Nonanoic acid and cholecystokinin induce beige adipogenesis.

Food science and biotechnology·2025
Same author

AuAgCu trimetallic nanoparticles based alloy: an advanced electrocatalyst for hydrogen evolution reaction in alkaline media.

RSC advances·2024
Same author

MLPH is a novel adipogenic factor controlling redox homeostasis to inhibit lipid peroxidation in adipocytes.

Biochemical and biophysical research communications·2024
Same journal

Redox-centric metabolic rewiring for dark-fermentative hydrogen production in Enterobacter aerogenes.

Bioprocess and biosystems engineering·2026
Same journal

Improving trehalose production through the design and optimization of linker peptides in scaffold protein.

Bioprocess and biosystems engineering·2026
Same journal

Elicitors derived from endophytic fungus Acremonium sp. enhance triterpenoid and polysaccharide production in the submerged cultivation of the medicinal mushroom Inonotus obliquus.

Bioprocess and biosystems engineering·2026
Same journal

Comparison of three inoculum sources for acetate production and microbial succession in H<sub>2</sub>/CO<sub>2</sub>-fed anaerobic system.

Bioprocess and biosystems engineering·2026
Same journal

Integrated cellulase continuous production and downstream processing using a packed-bed bioreactor for solid-state fermentation by thermophilic fungus.

Bioprocess and biosystems engineering·2026
Same journal

Eco-friendly synthesis of ZnO nanostructures from yeast strains isolated from kombucha and beetroot kwass for antimicrobial thin film applications.

Bioprocess and biosystems engineering·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Electrochemical aptasensor for tetracycline detection.

Yoon-Jin Kim1, Yeon Seok Kim, Javed H Niazi

  • 1School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701, Republic of Korea.

Bioprocess and Biosystems Engineering
|August 25, 2009
PubMed
Summary
This summary is machine-generated.

A new electrochemical aptasensor detects tetracycline using a specific DNA aptamer. This sensor shows high selectivity and can identify tetracycline in various samples, aiding in quality control.

More Related Videos

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

Related Experiment Videos

Last Updated: Jun 20, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Bacterial Detection &amp; Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry
06:02

Determining the Thermodynamic and Kinetic Association of a DNA Aptamer and Tetracycline Using Isothermal Titration Calorimetry

Published on: August 23, 2022

Area of Science:

  • Analytical Chemistry
  • Biosensors
  • Molecular Recognition

Background:

  • Tetracycline antibiotics are widely used but can lead to resistance and environmental concerns.
  • Accurate and selective detection methods for tetracycline are crucial for monitoring and safety.
  • Aptasensors offer a promising platform for sensitive and specific analyte detection.

Purpose of the Study:

  • To develop and validate an electrochemical aptasensor for sensitive and selective tetracycline detection.
  • To investigate the aptasensor's performance in distinguishing tetracycline from its derivatives.
  • To assess the potential application of the aptasensor in real-world samples.

Main Methods:

  • Development of a single-stranded DNA (ssDNA) aptamer selective for tetracycline.
  • Immobilization of biotinylated ssDNA aptamer onto a streptavidin-modified screen-printed gold electrode.
  • Electrochemical analysis using cyclic voltammetry and square wave voltammetry to detect tetracycline binding.

Main Results:

  • The aptasensor demonstrated high selectivity for tetracycline, differentiating it from structurally similar compounds like oxytetracycline and doxycycline.
  • The sensor achieved a detection limit in the range of 10 nM to micromolar.
  • Successful detection of tetracycline in a mixture with its derivatives was achieved.

Conclusions:

  • The developed electrochemical aptasensor is a sensitive and selective tool for tetracycline detection.
  • This aptasensor shows potential for monitoring tetracycline in pharmaceutical preparations, food, and water.
  • The study highlights the utility of aptasensors in addressing antibiotic residue concerns.