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Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
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Postnatal expression of GAD67.

Christopher P Turner1, Emily Ware, Robert Stowe

  • 1Neurobiology and Anatomy, Wake Forest University School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157-1010, USA. cpturner@wfubmc.edu

Neurochemical Research
|August 25, 2009
PubMed
Summary
This summary is machine-generated.

Glutamic acid decarboxylase 67 (GAD67) expression increases significantly in rat brains from postnatal day 7 to 21. Adult-like GAD67 expression develops late in the postnatal period, impacting GABAergic marker levels.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • N-methyl-D-aspartate receptor blockade is linked to apoptosis and altered glutamic acid decarboxylase 67 (GAD67) expression in developing brains.
  • Understanding the postnatal regulation of GAD67 is crucial for comprehending its role in neurodevelopment and potential links to excitotoxicity.

Purpose of the Study:

  • To investigate the developmental trajectory of GAD67 protein expression in the rat brain during the early postnatal period.
  • To characterize the temporal and spatial emergence of GAD67 expression from postnatal day 7 (P7) to P21.

Main Methods:

  • Western blot analysis or immunohistochemistry was used to detect and quantify GAD67 protein levels in rat brain tissues.
  • Brain samples were collected at three critical postnatal time points: P7, P14, and P21.
  • Expression patterns were analyzed in both puncta and cell bodies to assess cellular localization.

Main Results:

  • GAD67 protein levels showed a significant, age-dependent increase from P7 to P21, with up to a 6-fold increase by P21 compared to P7.
  • A notable shift in GAD67 localization was observed, with expression predominantly in puncta at P7 and robust labeling in both puncta and cell bodies by P14 and P21.
  • Regional variations in GAD67 expression levels were noted, with increases of 1.5- to 2-fold between P14 and P21.

Conclusions:

  • Adult-like GAD67 expression, a key GABAergic marker, is established late in the postnatal developmental period in rats.
  • The developmental increase in GAD67 expression and its cellular localization suggest a critical window for GABAergic system maturation.
  • These findings provide foundational insights into the regulation of GABAergic neurotransmission during brain development.