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Published on: October 15, 2018

LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer

Yan Wang1, Hua Zhang, Yupeng Chen

  • 1Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China.

Cell
|August 26, 2009
PubMed

Insights

Lysine-specific demethylase 1 (LSD1) is part of the NuRD complex, regulating cell pathways and inhibiting breast cancer invasion. LSD1 is downregulated in breast cancer, correlating with increased TGFbeta1 signaling.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Cancer Research

Background:

  • Lysine-specific demethylase 1 (LSD1) plays a role in development and cancer.
  • The Mi-2/nucleosome remodeling and deacetylase (NuRD) complex is involved in gene regulation.
  • Understanding the interplay between histone modification complexes is crucial for cancer research.

Purpose of the Study:

  • To investigate the role of LSD1 within the NuRD complex.
  • To determine the functional impact of LSD1/NuRD on cellular signaling pathways, particularly in breast cancer.
  • To explore the correlation between LSD1 expression and breast cancer progression.

Main Methods:

  • Transcriptional target analysis to identify regulated pathways.
  • In vitro assays to assess breast cancer cell invasion.
  • In vivo studies to evaluate metastatic potential.
  • Immunohistochemical analysis of LSD1 expression in breast carcinomas.

Main Results:

  • LSD1 was identified as an integral component of the NuRD complex.
  • The LSD1/NuRD complex regulates TGFbeta1 signaling, impacting cell proliferation, survival, and epithelial-to-mesenchymal transition.
  • LSD1 inhibited breast cancer cell invasion in vitro and suppressed metastatic potential in vivo.
  • LSD1 expression was downregulated in breast carcinomas and negatively correlated with TGFbeta1 levels.

Conclusions:

  • LSD1's integration into the NuRD complex expands its chromatin remodeling capabilities.
  • The LSD1/NuRD complex plays a significant role in suppressing breast cancer metastasis.
  • Downregulation of LSD1 in breast cancer may contribute to tumor progression via TGFbeta1 signaling.

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