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Related Concept Videos

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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Replication in Prokaryotes

Overview
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
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Related Experiment Video

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Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins
08:14

Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

Published on: February 25, 2017

Transposition into replicating DNA occurs through interaction with the processivity factor.

Adam R Parks1, Zaoping Li, Qiaojuan Shi

  • 1Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.

Cell
|August 26, 2009
PubMed
Summary
This summary is machine-generated.

The bacterial transposon Tn7 uses protein TnsE to target replicating DNA. TnsE interacts with DNA replication machinery, guiding transposition to active replication sites.

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Last Updated: Jun 20, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • The bacterial transposon Tn7 exhibits a preference for inserting into actively replicating DNA.
  • This transposition is mediated by the transposon-encoded protein TnsE.

Purpose of the Study:

  • To investigate the interaction between TnsE and the host DNA replication machinery.
  • To elucidate the mechanism by which Tn7 targets actively replicating DNA.

Main Methods:

  • In vivo and in vitro biochemical assays to study protein-protein interactions.
  • Reconstitution of the TnsABC+E transposition reaction using purified proteins and DNA.
  • Analysis of transposition site selection in relation to DNA replication.

Main Results:

  • TnsE physically and functionally interacts with the DNA replication processivity factor.
  • An in vitro transposition system (TnsABC+E) was successfully reconstituted.
  • The processivity factor was shown to reorder TnsE-mediated transposition events, mirroring in vivo replication bias.

Conclusions:

  • TnsE's interaction with replication machinery is key to Tn7's target site selection.
  • This mechanism allows Tn7 to preferentially transpose into actively replicating DNA.
  • Similar mechanisms may be employed by other mobile genetic elements.