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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Cell Motility through Blebbing01:16

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Related Experiment Video

Updated: Jun 20, 2026

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
10:15

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

Enigmatic brefeldin a.

Yu Chung Tse1, Sheung Kwan Lam, Liwen Jiang

  • 1Department of Biology and Molecular Biotechnology Program; The Chinese University of Hong Kong, Shatin, New Territories; Hong Kong, China.

Plant Signaling & Behavior
|August 26, 2009
PubMed
Summary
This summary is machine-generated.

Brefeldin A (BFA) impacts plant cell organelles, revealing distinct sensitivities of Golgi and prevacuolar compartments (PVCs). This finding offers a new tool for studying PVC protein sorting and biogenesis.

Keywords:
BY-2 cellsbrefeldin A (BFA)endosomal compartmentmultivesicular bodies (MVB)prevacuolar compartment (PVC)vacuolar sorting receptor (VSR)wortmannin

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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
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Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
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Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes

Published on: October 3, 2019

Area of Science:

  • Plant Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Brefeldin A (BFA), a fungal macrocyclic lactone, is a key tool for investigating protein trafficking in plant secretory and endocytic pathways.
  • Transgenic plant cells with GFP-tagged organelle markers enable dynamic studies of organelle responses to BFA.
  • Previous studies show BFA induces morphological changes in the endoplasmic reticulum (ER), Golgi apparatus, and endosomal compartments, forming aggregates or ER-Golgi hybrids.

Purpose of the Study:

  • To investigate the differential sensitivity of Golgi apparatus and prevacuolar compartments (PVCs) to BFA in plant cells.
  • To extend the known actions of BFA to plant PVCs/multivesicular bodies (MVBs).
  • To establish BFA as a tool for studying PVC-mediated protein sorting and biogenesis.

Main Methods:

  • Utilized transgenic tobacco BY-2 cells.
  • Employed membrane-anchored yellow fluorescent protein (YFP) reporters to mark Golgi apparatus and PVC.
  • Observed organelle morphology and aggregation in response to BFA treatment at high concentrations (50-100 microg/ml).

Main Results:

  • Demonstrated differential sensitivity of Golgi and PVC organelles to BFA.
  • Showed that high concentrations of BFA induce aggregation of PVCs or multivesicular bodies (MVBs).
  • Extended the known effects of BFA to include plant PVCs/MVBs.

Conclusions:

  • BFA affects plant PVCs/MVBs, inducing aggregation at high, recoverable concentrations.
  • BFA is a valuable tool for studying PVC-mediated protein sorting and PVC biogenesis in plants.
  • The differential sensitivity of organelles to BFA provides insights into protein trafficking pathways.