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Improving biomedical imaging with gold nanocages

Younan Xia1, Sara E Skrabalak

  • 1Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO.

SPIE Newsroom
|September 28, 2011
PubMed

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.