Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma GDF15 affects long-term dementia risk and alters neuroimmune signaling.

Science advances·2026
Same author

Establishing an observational framework for the objective evaluation of parent training effectiveness.

Journal of family medicine and primary care·2026
Same author

The Benefits of the Positive Parenting Program as Early Intervention for Mothers of Children Aged 1-3 Years Who May Have Neurodevelopmental Disorders.

Children (Basel, Switzerland)·2026
Same author

OMG! A proteomic determinant of neurodegenerative resiliency.

Molecular neurodegeneration·2026
Same author

Plasma GDF15 affects long-term dementia risk and alters neuro-immune signaling.

medRxiv : the preprint server for health sciences·2025
Same author

Clinical and plasma proteomic characterization of heart failure with supranormal left ventricular ejection fraction: An emerging entity of heart failure.

European journal of heart failure·2025

Related Experiment Video

Updated: Jun 20, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Antibody-specific aptamer-based PCR analysis for sensitive protein detection.

Yoshihito Yoshida1, Katsunori Horii, Nobuya Sakai

  • 1VALWAY Technology Center, NEC Soft, Ltd, Tokyo, 136-8627, Japan.

Analytical and Bioanalytical Chemistry
|August 26, 2009
PubMed
Summary

A new immuno-aptamer PCR (iaPCR) method enhances ELISA sensitivity by over 100 times using an RNA aptamer. This cost-effective technique simplifies detection for antibody-based assays, offering a sensitive alternative to traditional methods.

More Related Videos

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Related Experiment Videos

Last Updated: Jun 20, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
11:14

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
10:46

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Assay Development

Background:

  • Enzyme-linked immunosorbent assay (ELISA) is a common immunoassay.
  • Immuno-polymerase chain reaction (immuno-PCR) enhances ELISA sensitivity but requires oligonucleotide-labeled antibodies.
  • There is a need for more sensitive and cost-effective immunoassay methods.

Purpose of the Study:

  • To develop a novel detection system, immuno-aptamer PCR (iaPCR), by combining ELISA with nucleic acid amplification.
  • To improve the sensitivity of antibody-based ELISA systems without requiring oligonucleotide-labeled antibodies.
  • To establish a cost-effective and easy method for highly sensitive immunoassays.

Main Methods:

  • Utilized an RNA aptamer specific for rabbit IgG as a replacement for the secondary antibody in a standard ELISA protocol.
  • After washing the assay plate, quantitative reverse transcription (RT)-PCR was performed for detection.
  • The method, termed immuno-aptamer PCR (iaPCR), does not require ribonuclease (RNase) inhibitors.

Main Results:

  • The iaPCR method demonstrated a detection limit over 100 times more sensitive than conventional ELISA.
  • Successfully detected 16 attomoles (16 x 10(-18)) of vascular endothelial growth factor (VEGF).
  • A small amount of aptamer (1 mg) is sufficient for over 10 million assays, indicating cost-effectiveness.

Conclusions:

  • Immuno-aptamer PCR (iaPCR) offers a significant increase in sensitivity compared to traditional ELISA.
  • This method is a cost-effective and simplified approach to enhance antibody-based immunoassay sensitivity.
  • iaPCR provides a valuable alternative for sensitive detection in various biological applications, distinct from conventional immuno-PCR.