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Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Published on: August 25, 2009

Antifouling self-assembled monolayers on microelectrodes for patterning biomolecules.

John Noel¹, Winfried Teizer, Wonmuk Hwang

¹Department of Physics, Texas A & M University, TX, USA.

Journal of Visualized Experiments : Jove

|August 27, 2009

Summary

We developed a new method to coat microelectrodes with poly(ethylene glycol) (PEG) self-assembled monolayers (SAMs) for effective biomolecule patterning. This technique uses electrophoresis to immobilize molecules, preventing biofouling and enabling nanoscale patterning.

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Area of Science:

Materials Science
Surface Chemistry
Biotechnology

Background:

Biofouling on silicon and gold surfaces complicates biosensor development.
Patterning biomolecules directly onto surfaces is challenging using traditional lithography.
Self-assembled monolayers (SAMs) offer a route to functionalize surfaces for biomolecule immobilization.

Purpose of the Study:

To present a novel procedure for creating poly(ethylene glycol) (PEG) trimethoxysilane SAMs on silicon with gold microelectrodes.
To demonstrate a biofouling-resistant coating for microelectrodes.
To establish a generalizable method for biomolecule patterning using electrophoresis.

Main Methods:

Formation of PEG-SAMs on silicon substrates with gold microelectrodes in a single assembly step.
Coating microelectrodes patterned via standard, positive-tone lithography.
Inducing electrophoretic migration of microtubules to SAM-coated electrodes using DC voltage.
In situ imaging of electrophoretic migration and patterning using epifluorescence microscopy within a flow chamber.

Main Results:

Successful formation of a biofouling-resistant PEG-SAM on silicon and gold surfaces.
Reversible electrophoretic immobilization of microtubules onto SAM-coated electrodes.
Demonstration of in situ imaging of biomolecule patterning via epifluorescence microscopy.
Successful application of the method for nanoscale biomolecule patterning, compatible with electron beam lithography.

Conclusions:

The developed PEG-SAM coating procedure effectively prevents biofouling on microelectrodes.
Electrophoretic immobilization is a versatile technique for biomolecule patterning, independent of specific molecular interactions.
This method offers a robust and scalable approach for nanoscale biomolecule patterning on microelectronic devices.