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Published on: August 13, 2019
Maxillary sinus floor elevation using a tissue-engineered bone complex with beta-TCP and BMP-2 gene-modified bMSCs in
1Oral Bioengineering Lab, Shanghai Research Institute, Shanghai Key Lab of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Clinical Oral Implants Research
|August 28, 2009
Summary
Bone marrow stromal cells (bMSCs) modified with bone morphogenetic protein-2 (BMP-2) gene significantly enhanced bone regeneration in rabbit maxillary sinus elevation. This tissue engineering approach shows promise for bone augmentation procedures.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Oral and Maxillofacial Surgery
Background:
- Maxillary sinus floor elevation is crucial for dental implant placement.
- Tissue-engineered bone complexes offer potential for bone regeneration.
- Gene modification of stromal cells can enhance osteogenesis.
Purpose of the Study:
- To evaluate the efficacy of a tissue-engineered bone complex using beta-tricalcium phosphate (beta-TCP) scaffold and bone morphogenetic protein-2 (BMP-2) gene-modified bone marrow stromal cells (bMSCs) for maxillary sinus floor elevation in rabbits.
Main Methods:
- Autologous rabbit bMSCs were transduced with adenovirus carrying BMP-2 (AdBMP-2) or enhanced green fluorescent protein (AdEGFP).
- Gene-modified bMSCs were combined with beta-TCP scaffolds and implanted for maxillary sinus elevation in rabbits.
- Histological and histomorphometric analyses were performed at 2 and 8 weeks post-surgery.
Main Results:
- AdBMP-2 modified bMSCs significantly increased new bone formation compared to controls.
- At 8 weeks, the BMP-2 group exhibited the largest bone area and mature bone structure.
- EGFP expression in newly formed bone suggested bMSC contribution to osteogenesis.
Conclusions:
- BMP-2 gene-modified bMSCs effectively promote bone formation and maturation in maxillary sinus elevation.
- Regional gene therapy combined with tissue engineering is a viable strategy for bone regeneration in the maxillary sinus.
