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Updated: Jun 20, 2026

Agrobacterium tumefaciens-Mediated Genetic Engineering of Green Microalgae, Chlorella vulgaris
Published on: October 27, 2023
Plasmid localisation of atrazine-degrading genes in newly described Chelatobacter and Arthrobacter strains
Sandrine Rousseaux1, Guy Soulas, Alain Hartmann
1INRA UMR Laboratoire de Microbiologie des Sols/Géosol, 17 rue Sully, P.O. Box 86510, 21065 Dijon Cedex, France.
Abstract:
Abstract In a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. The aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. In the case of the Gram-negative strains of Chelatobacter heintzii, six to seven plasmids were observed. The atzABC and trzD genes were located on two or three plasmids with variable molecular masses. For the Gram-positive strains of Arthrobacter crystallopoietes, the atzBC genes were located on a single plasmid of 117 kb. The organisation of atrazine-degrading genes seems to be highly variable between the strains studied. We have shown by a specific PCR the occurrence of IS1071-like sequences in all strains carrying atzBC genes, while these sequences seem to be absent in strains carrying only the atzA gene. Thus, we show a strong correlation between the presence of these insertion sequences and the presence of some genes involved in atrazine degradation.
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