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Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles - a critique
Juliet Preston-Mafham1, Lynne Boddy, Peter F Randerson
1Cardiff School of Biosciences, University of Wales Cardiff, P.O. Box 915, Cardiff CF10 3TL, UK.
FEMS Microbiology Ecology
|August 28, 2009
Summary
This study reviews the BIOLOG bacterial identification system for assessing microbial functional diversity. It critically evaluates the method
Area of Science:
- Microbiology
- Environmental Science
Background:
- Understanding microbial functional diversity (metabolic potential) is crucial for ecological studies.
- The BIOLOG bacterial identification system is widely used to assess microbial metabolic potential using carbon source utilization patterns.
Purpose of the Study:
- To summarize the basis of the BIOLOG system approach.
- To critically assess the pros and cons, biases, limitations, and methodological difficulties of the BIOLOG system.
- To suggest potential solutions for overcoming identified challenges.
Main Methods:
- Review of the BIOLOG system methodology, including plate types, sample treatment, replication, incubation, monitoring, and statistical analysis.
- Critical assessment of the benefits and drawbacks of the BIOLOG approach.
- Identification of inherent biases and limitations.
Main Results:
- The BIOLOG system offers a broad assessment of microbial metabolic potential.
- Significant challenges and limitations exist within the BIOLOG methodology.
- Methodological improvements and careful consideration of biases are necessary for accurate results.
Conclusions:
- While valuable, the BIOLOG system requires careful application and interpretation.
- Addressing methodological difficulties is key to maximizing the utility of BIOLOG for microbial functional diversity assessment.
- Further research into optimizing protocols can enhance the reliability of this technique.
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