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Related Concept Videos

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics
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Published on: October 5, 2016

Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development.

Fiammetta Alagna1, Nunzio D'Agostino, Laura Torchia

  • 1CNR-Institute of Plant Genetics, Via Madonna Alta 130, 06128 Perugia, Italy.

BMC Genomics
|August 28, 2009
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Summary

Genomic information for olive trees (Olea europaea) was expanded using 454 pyrosequencing to identify genes related to fruit quality. This research provides insights into the olive tree transcriptome and phenolic content regulation.

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Area of Science:

  • Plant genomics
  • Molecular biology
  • Agricultural science

Background:

  • Genomic information for the economically important olive tree (Olea europaea) is limited.
  • This study aimed to enrich existing sequence data and identify genes related to fruit quality traits.

Purpose of the Study:

  • To characterize the olive tree transcriptome during fruit development.
  • To identify genes influencing fruit quality and phenolic content.

Main Methods:

  • Utilized 454 pyrosequencing to analyze cDNA from two olive varieties (Coratina and Tendellone) at different drupe development stages.
  • Generated over 261,000 reads (58 Mb) and processed data using a four-step pipeline.
  • Stored data in a relational database with a web interface.

Main Results:

  • Sequenced four cDNA libraries from olive fruits, yielding substantial transcriptomic data.
  • Identified differentially expressed genes through comparative transcript profiling.
  • The data provides large-scale information on gene transcripts during fruit development.

Conclusions:

  • Massively parallel sequencing offers valuable insights into olive tree gene structure and function.
  • Comparative transcript profiling successfully identified genes potentially regulating fruit metabolism and phenolic content during ripening.