Sequential course of uptake of intravitreal 5,7-dihydroxytryptamine by carp retinal cells

The sequential course of uptake by retinal cells of intravitreally injected 5,7-dihydroxytryptamine (5,7-DHT) together with dopamine (DA) was investigated in juvenile carp retinas, which were removed at various intervals (1-24 h) after injection. The cells taken up 5,7-DHT were visualized immunohistochemically with anti-serotonin (5-HT) antibody and FITC-conjugated IgG. After a mixture of 5,7-DHT and DA (2.5, 10 or 20 micrograms each) was given, large-sized indoleamine (IA) amacrine cells first (1-4 h), and then small-sized indoleamine-accumulating amacrine amacrine (IAA) cells (4-12 h), bipolar cells (8-12 h) and in some cases photoreceptor cells (12-24 h) were sequentially observed, and finally the immunoreactive structures almost disappeared around 24 h after injection. When the mixture of 5,7-DHT and DA (10 micrograms each) was injected into the eyes of reserpinized fish, the same sequential uptake of 5,7-DHT was seen in a faster time course, but additionally various classes of retinal cells (horizontal, ganglion and Müller cells) became visible as irregular clusters. However, DA cells were never visualized at any stages of all the experiments, indicating that DA cells do not take up 5,7-DHT in the carp retina, which was further confirmed by double labeling of 5-HT- and tyrosine hydroxylase-like immunoreactive cells. Double labeling also revealed that 5,7-DHT-accumulating bipolar cells appear to represent a subclass different from that of protein kinase C-like immunoreactive bipolar cells.