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Related Concept Videos

The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

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Related Experiment Video

Updated: Jun 20, 2026

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Engineering next generation proteases.

Mark Pogson1, George Georgiou, Brent L Iverson

  • 1Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX 78712, United States.

Current Opinion in Biotechnology
|August 28, 2009
PubMed
Summary
This summary is machine-generated.

Researchers are engineering proteases with novel sequence specificity for analytical, biotechnological, and therapeutic applications. Advances in high-throughput assays enable reprogramming proteases for new substrate cleavage, paving the way for engineered protease tools.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Protein Engineering
  • Biotechnology

Background:

  • Proteases are enzymes crucial in biological processes.
  • Engineering protease specificity is key for developing new biotechnological and therapeutic tools.
  • Existing proteases have limitations in substrate specificity.

Purpose of the Study:

  • To engineer novel and precise sequence specificity into proteases.
  • To develop new tools for analytical, biotechnological, and therapeutic applications using engineered proteases.

Main Methods:

  • Utilized high-throughput assay technologies for isolating protease variants from large libraries.
  • Employed directed evolution and other approaches to reprogram protease specificity.
  • Focused on altering cleavage sites at the P1 and P1' positions.

Main Results:

  • Successfully reprogrammed proteases to cleave substrates with diverse amino acids at P1 and P1' positions.
  • Achieved cleavage specificity for a post-translationally modified tyrosine, a feature not found in natural proteases.
  • Demonstrated substantial progress in engineering protease specificity.

Conclusions:

  • Engineered proteases with novel specificities can be developed using advanced screening technologies.
  • These reprogrammed proteases offer new possibilities for analytical, biotechnological, and therapeutic applications.
  • Recent advances suggest widespread application of engineered proteases is imminent.