Increasing the endothelial layer permeability through ultrasound-activated microbubbles
Klazina Kooiman1, Marcia Emmer, Miranda Foppen-Harteveld
1Department of Biomedical Engineering, ErasmusMC, P.O. Box 2040, Rotterdam 3000 CA, The Netherlands. k.kooiman@erasmusmc.nl
IEEE Transactions on Bio-Medical Engineering
|August 28, 2009
Summary
Ultrasound and BR14 microbubbles safely increase endothelial layer permeability, enhancing drug delivery efficiency. This method shows promise for targeted ultrasound-guided therapeutic applications.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Pharmacology
Background:
- Vascular endothelial permeability is crucial for efficient drug delivery to diseased tissues.
- Ultrasound combined with contrast agents can increase cell membrane permeability.
- The effect of ultrasound and contrast agents on endothelial layer integrity is not well understood.
Purpose of the Study:
- To investigate the feasibility of increasing endothelial layer permeability using ultrasound and a contrast agent without causing cell damage.
- To assess the impact of ultrasound-activated BR14 microbubbles on endothelial barrier function.
Main Methods:
- Monolayers of human umbilical vein endothelial cells were treated with ultrasound and the BR14 contrast agent.
- Transendothelial electrical resistance (TEER) was measured to assess barrier function.
- Cellular integrity was evaluated post-treatment to detect damage or detachment.
Main Results:
- Ultrasound-activated BR14 significantly decreased TEER by 40.3% (p < 0.01), indicating increased permeability.
- No significant cell detachment or damage was observed after the treatment.
- The findings demonstrate controlled enhancement of endothelial layer permeability.
Conclusions:
- Ultrasound-activated BR14 microbubbles effectively increase endothelial layer permeability.
- This technique offers a potential method for controlled drug delivery enhancement.
- The approach is suitable for developing future ultrasound-guided drug delivery systems.
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